FastQCFastQC Report
Wed 23 Jul 2014
sample.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamesample.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19485100
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCGACATGGCTTCGACGTGGGTAGCGAAACCTTAAGCGCATGCCGA6006573.08264776675511No Hit
TGAAAGAGTCGGCTGGACAGTCGCTGTTTTATCGCAAGATAAGATGGAGG4002522.054143935622604No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3437241.7640350832174327TruSeq Adapter, Index 1 (100% over 49bp)
TGGGGAGTAATTGTCTTCCCAATTATTAATTTTCAGACAGCGTCTGGGAA3238881.6622342199937388No Hit
GAAAGAGTCGGCTGGACAGTCGCTGTTTTATCGCAAGATAAGATGGAGGA1674730.8594926379643933No Hit
GGGGAGTAATTGTCTTCCCAATTATTAATTTTCAGACAGCGTCTGGGAAT935900.4803157284283889No Hit
GGGGGGCGACATGGCTTCGACGTGGGTAGCGAAACCTTAAGCGCATGCCG865930.4444062386130941No Hit
AGCTTGAAATAGTTTTACCGTTATGCTTATCTCTACGAAGTTCTTCATGT837370.4297488850454963No Hit
TTGATCGATGGGGTGACAGGAATTGGGCCGCGGTGTCAGCGTATGCTGAC702900.36073717866472327No Hit
GAGACGAAGGTTTAACAGGTTTTTTGAATACATAAATTAATTTTTTCTCT514820.2642121415851086No Hit
GCATAAAGAGTAAGTAACTAAAATTATAACTATTACTATAACCCATCGGA492300.2526545924834874No Hit
TAGTAAGCGTTTTTTTGTTGTAATAACTTTAAGAAGGAAAAATAATATGA476170.2443764722788182No Hit
GGAAGATGGGCTTTGTTGTATTAGAGCAAATTTTTATAAAATAACAACCT409700.21026322677327805No Hit
TCACTAAATTGACTTGGTGGCACTTGGGCCTTAATGTTTTTAGGTTTGGG371800.19081246696193502No Hit
ATCGACCGATGAAATGCTGTTGATTATGTCCTGGATACGCGACATCTATA353270.18130263637343405No Hit
GAGCAAGTTTGCTTAAATAATAATAATTACCCACACAACTGGAGACAAAT303880.15595506309949653No Hit
AGTTAGAGTTTGAGCCGGCTTTATTATGAAGCTGGGATTTGTAACGCCGA277040.14218043530697816No Hit
TTTGATCGATGGGGTGACAGGAATTGGGCCGCGGTGTCAGCGTATGCTGA249770.1281851260706899No Hit
TGGGGGCGACATGGCTTCGACGTGGGTAGCGAAACCTTAAGCGCATGCCG231700.1189113733057567No Hit
GGCATCAGCCTTTCATTATGTTGAATTCTTGCTTTTTTGCTTAAATTCTC216940.11133635444519145No Hit
AAAATGAAAAAGATATTGAGGAATTTGACATGGCTATCGAAATAACCGGC213290.10946312823644734No Hit
TCGGCATCAGCCTTTCATTATGTTGAATTCTTGCTTTTTTGCTTAAATTC212040.10882161241153497No Hit
GGTGTAGGGCTATTATCATTAATAGTTACACAGCCAATTTAATTAAAAAA207690.10658913734083993No Hit
CGTGGGTAGCGAAACCTTAAGCGCATGCCGAGGACAGTACACCTCGTAAA206240.10584497898394159No Hit
GAACTGGTTTTAGTTTGGCTGCCGGTTAACTGAAAGTGTTATGTTTCGTA197070.10113881889238445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGAG377500.082.723661
GATCGGA532700.074.3334352
AGAGCAC543300.071.759749
GGGGGCG805750.071.705691
TCGGAAG558050.071.2269744
AGATCGG563300.070.185081
ATCGGAA587150.067.873433
GGGCGAC866250.067.313073
GGGGCGA869950.066.5067752
GGAGTAA488700.065.954784
CGACATG908250.064.756936
TGAAAGA605850.064.438311
GACATGG914350.064.3554157
GGGAGTA498650.064.326883
ACATGGC926500.063.521618
CGGAAGA637800.062.255735
AAATGAC959550.062.20849294
GGGGAGT508750.062.1698842
GGCGACA952000.061.517244
GAGTAAT530350.061.054155
Produced by FastQC (version 0.11.2)