FastQCFastQC Report
Wed 30 Jul 2014
sample.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamesample.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18546469
Sequences flagged as poor quality0
Sequence length18-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCGACATGGCTTCGACGTGGGTAGCGAAACCTTAAGCGCATGCCGA5897283.1797319478980066No Hit
TGAAAGAGTCGGCTGGACAGTCGCTGTTTTATCGCAAGATAAGATGGAGG3906602.1063847786875227No Hit
TGGGGAGTAATTGTCTTCCCAATTATTAATTTTCAGACAGCGTCTGGGAA3203161.7270996436033188No Hit
GAAAGAGTCGGCTGGACAGTCGCTGTTTTATCGCAAGATAAGATGGAGGA1637280.8827987688653834No Hit
GGGGAGTAATTGTCTTCCCAATTATTAATTTTCAGACAGCGTCTGGGAAT926860.4997501141591965No Hit
GGGGGGCGACATGGCTTCGACGTGGGTAGCGAAACCTTAAGCGCATGCCG854750.4608693978352429No Hit
AGCTTGAAATAGTTTTACCGTTATGCTTATCTCTACGAAGTTCTTCATGT829800.44741670233832653No Hit
TTGATCGATGGGGTGACAGGAATTGGGCCGCGGTGTCAGCGTATGCTGAC693030.37367220682276503No Hit
GAGACGAAGGTTTAACAGGTTTTTTGAATACATAAATTAATTTTTTCTCT504420.271976299100384No Hit
GCATAAAGAGTAAGTAACTAAAATTATAACTATTACTATAACCCATCGGA482490.26015194590409635No Hit
TAGTAAGCGTTTTTTTGTTGTAATAACTTTAAGAAGGAAAAATAATATGA468720.2527273520366599No Hit
GGAAGATGGGCTTTGTTGTATTAGAGCAAATTTTTATAAAATAACAACCT401440.21645090502132777No Hit
TCACTAAATTGACTTGGTGGCACTTGGGCCTTAATGTTTTTAGGTTTGGG362640.19553048076159404No Hit
ATCGACCGATGAAATGCTGTTGATTATGTCCTGGATACGCGACATCTATA342970.18492468835981662No Hit
GAGCAAGTTTGCTTAAATAATAATAATTACCCACACAACTGGAGACAAAT295690.1594319651897081No Hit
AGTTAGAGTTTGAGCCGGCTTTATTATGAAGCTGGGATTTGTAACGCCGA251740.13573473203982925No Hit
TTTGATCGATGGGGTGACAGGAATTGGGCCGCGGTGTCAGCGTATGCTGA246520.13292018011622592No Hit
TGGGGGCGACATGGCTTCGACGTGGGTAGCGAAACCTTAAGCGCATGCCG228290.1230908158313046No Hit
GGCATCAGCCTTTCATTATGTTGAATTCTTGCTTTTTTGCTTAAATTCTC207970.11213455240455744No Hit
AAAATGAAAAAGATATTGAGGAATTTGACATGGCTATCGAAATAACCGGC205930.11103461257234463No Hit
TCGGCATCAGCCTTTCATTATGTTGAATTCTTGCTTTTTTGCTTAAATTC205680.11089981602427934No Hit
GGTGTAGGGCTATTATCATTAATAGTTACACAGCCAATTTAATTAAAAAA205240.11066257409968441No Hit
CGTGGGTAGCGAAACCTTAAGCGCATGCCGAGGACAGTACACCTCGTAAA202040.10893717828444865No Hit
GAACTGGTTTTAGTTTGGCTGCCGGTTAACTGAAAGTGTTATGTTTCGTA190550.1027419289353677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGAG364850.082.0630341
GGGGGCG777250.070.888531
AAATGAC902850.069.4756594
GGCGTGA263500.066.4736494
GGGCGAC844400.065.90113
GGGGCGA844350.065.318812
GGAGTAA479050.064.443724
TGAAAGA583350.064.248091
AGGCGTG629850.064.02746694
GGGGGGC131300.063.86751
CGACATG879350.063.7985086
GACATGG883800.063.586917
GGGAGTA488350.062.8565373
GGGGAGT485700.062.370922
ACATGGC905400.062.10568
GGCGACA925250.060.3575174
GAGTAAT520900.059.559055
GCGACAT945100.059.3503235
AGAGTCG667950.059.0258835
CATGGCT960150.058.4911659
Produced by FastQC (version 0.11.2)